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Inheritance of a variegated chlorophyll deficiency in diploid alsike clover (Trifolium hybridum L.), was determined under greenhouse conditions. The variegated character was first observed in the S2 progeny of a normal green Sj clone. The S2 progeny segregated 9 green: 7 variegated plants. Reciprocal Ft progenies of this S, clone and another Si clone whose S2 progeny was normal green in color, were green also. The F2 data fit phenotypic ratios of 9 green: 7 variegated plants or 3 green: 1 variegated plant depending upon the genotype of the Ft parent. F2 intercross progenies were grown to confirm the F3 segregations and to assign genotypes to several F2 plants.
Two recessive genes controlled the chlorophyll deficiency. The homozygous recessive conditions for either pair or both pairs of allcles produced the variegated character and the gene symbols va and vs were suggested for the two loci.
Key Words: Trifolium hybridum L. Variegation
2 Former graduate research assistant, Dep. of Agron., Colo. State Univ. (now corn breeder, Pioner Hi-Bred Int., Inc., York, NE 68647): and research geneticist, ARS, USDA, crops res. lab., Colo. State Univ., Fort Collins, CO 80523.
Received for publication November 22, 1974.
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