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Published in Crop Sci 15:550-553 (1975)
© 1975 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Studies on Male Sterility in Barley Induced by Sodium 1-(p-Chlorophenyl) 1,2-Dihydro-4,-6-Dimethyl-2-Oxonicotinate1

R. C. Wang and Steve Lund2

Foliar sprays with various rates of an aqueous solution of sodium 1-(p-chlorophenyl) 1,2-dihydro-4,6-dimethyl-2- oxonicotinate (RH-531) were applied to barley (Hordeum vulgate L.) plants at different stages of growth to find the optimum application time and dosage for induction of complete male sterility. Under greenhouse conditions, 1.5 kg/ha a.i. of RH-531 applied several days before meiosis in pollen mother cells (about six.leaf stage or 20 days before heading) induced complete male sterility. At least 19% female fertility was present on RH-531. induced male-sterile ‘Barsoy’ plants, estimated by natural pollination. Female fertility of RH-531.induced malesterile ‘Larker’ barley was 40% of the control when both were hand pollinated.

Meiosis in pollen mother cells did not appear to be affected by RH-531. Paraffin sections of anthers from RH-531-induced lnale-sterile barley revealed abnormal persistence of the tapetum. Acetocarmine was inferior to iodine-potassium iodide (IKI) and Alexander's multiple stain for determining pollen viability in RH-531- treated barley plants. Staining of pollen grains with IKI suggested that pollen sterility induced by RH-531 was related to an absence of or a decrease in carbohydrates.

Positive correlations were found between percentage seed-set of bagged spikes and anther length, and between percentage seed-set of bagged spikes and pollen grain carbohydrate content. It appeared that RH-531 inhibited anther growth thus leading to either degeneration of microspores or reduction in carbohydrates in pollen grains.

Key Words: Meiosis • Pollen • Tapetum • Pollination • Female fertility • Anthers • Gametocide • Hybrid barley • Hordeum vulgare L. • RH-531

1 Paper of the journal series, N. J. Agric. Exp. Stn., Cook Coll., Rutgers Univ.—The State Univ. of N. J., New Brunswick, NJ 08903. Submitted by the senior author in partial fulfillment of the requirements for the Ph.D. degree. This research was supported in part by grants from the Kohm and Haas Co. and Hatch funds.

2 Research intern and research professor, respectively. Soils and Crops Dep.

Received for publication December 5, 1974.




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