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Variability in Metabolism of Individual Axes of Soybean Seeds and Its Relationship to Vigor¹

Vigor was measured on individual seeds of soybean (Glycine max L. ‘Lee-68’) by an isotopic method for determining total uptake, incorporation and leaching of ¹⁴C-leucine fed to the individual axes. Results were similar with ³²P, which reduced the time necessary for a determination because uptake of ³²P by the axis was measured without extracting the tissue.

The isotopic and conductivity methods for determining leachates were compared. More sugars and amino acids were leached from deteriorated than from vigorous seeds, whether the components were derived from an endogenous source, (e.g., stored food reserves) or from the incubation medium.

Key Words: Leguminaceae • Soybean • Embryonic axes • Glucose metabolism • Amino acid metabolism • ³²P • Leaching • Conductance

² Plant physiologists, seed qual. lab. and post harvest plant physiol. lab., respectively, ARS, USDA, Beltsville, MD 20705

Received for publication October 4, 1974.