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Seed of genetic selection M-8 (Gossypiura hirsutum L.) and Pima S-4 (G. barbademe L.) were subjected to various levels of chilling (0, 1, 2, 4, 8, 16, and 32 days at 5 C in water) after the seed were preconditioned by inbibition for 6 hours at 30 C in water. Seed for evaluation of chilling injury were germinated over a temperature range of 15 to 30 C on a thermogradient plate and in rolled towels at 30 C. Percent emergence and stand survival were determined for both cultivars.
Higher percent germination over the same range of temperatures on the thermogradient plate was obtained with M-8 than with Pima S-4. Seed chilled for 16 and 32 days was very low in viability and germinated over a narrower range of temperatures than seed chilled for shorter periods. Preconditioning increased germination of both selections and improved emergence of M-8. Seed of Pima S.4 inbibed water at a faster rate than M-8. Selections differed in their responses to different durations of chilling.
Key Words: Thermogradient plate Emergence Seedling Gossypium hlrsutum L. G. barbadense
2 Plant physiologist, ARS, USDA, N. Dak. State U., Fargo, ND 58102 (formerly research plant physiologist, seed quality lab., AMRI, ARS, USDA, Beltsville, MD 20705) and plant physiologist and leader, plant stress lab., PPHI, ARS, USDA, Beltsville, MD 20705
Received for publication September 25, 1974.
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