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Evaluation of Alfalfa for Stem-Nematode Resistance¹

An improved laborsaving technique for evaluating stem-nematode [Ditylenchus dipsaci (Kühn) Filipjev] resistance in alfalfa (Medicago sativa L.) was developed. To demonstrate the technique, seedlings of three resistant and three susceptible varieties were inoculated by watering with nematode suspensions at seeding and again at 2, 4, and 6 weeks and were rated 16 weeks after seeding. Resistant varieties ‘Apalachee,’ ‘Lahontan,’ and ‘Washoe’ averaged 90.1, 67.8, and 63.7% resistant plants, respectively. Susceptible varieties ‘Saranac,’ ‘Vernal,’ and ‘Ranger’ averaged 34.9, 15.1, and 12.2% resistant plants. Because of its ease of application and its ability to differentiate clearly between resistant and susceptible varieties, this method should appeal to many workers.

Key Words: Ditylenchus dipsaci • Medicago sativa • Screening • Breeding

² Research agronomist, field crops lab., ARS, USDA, Beltsville, MD 20705 (formerly, Irrigated Agr. Res. and Ext. Cen., Pressey, Wash.); associate agronomist; and former nematologist (now head, Dept, of Plant Pathol., Kans. State U., Manhattan), Wash. State U., IAREC, Prosser, WA 99350, respectively.

Received for publication September 27, 1974.