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Published in Crop Sci 14:891-893 (1974)
© 1974 Crop Science Society of America
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Anther Culture in Soybeans1

D. R. Ivers, R. G. Palmer and W. R. Fehr2

To facilitate anther culture in soybeans [Giycine max (L.) Merr.], five bud stages from the cultivar ‘Hark’ are described. Combinations of bud length, corolla development, and anther development were indicators used to classify buds as stages 1 through 5, Stage 3 contained microspores that were predominantly individualized, uninucleate, and devoid of starch. Culture contamination, which ranged from 3.3% with buds from the winter greenhouse, to 100% with material from the field, was best controlled by treatment with 95% ethanol for 2 min, 5.25%N aOCfIo r 5 min followed by a sterile water rinse. Miller's and Nitsch's basic media are evaluated for callus growth and plantlet development, with addition of various levels of coconut milk, kinetin, iron, and auxins. No embryoids or plantlets were observed and. the callus formed was diploid.

Key Words: Bud staging • Bud sterilization • Differentiation • Glycine max (L.) Merr.

1 Joint contribution: Journal Paper No. J-7862 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project 1179; and ARS, USDA. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA or Iowa State University and does not imply its approval to the exclusion of other products that may also be suitable.

2 Research Associate; Research Geneticist, ARS, USDA; and Professor; respectively, Department of Agronomy, Iowa State University, Ames, IA 50010.

Received for publication April 20, 1974.




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