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Published in Crop Sci 14:854-856 (1974)
© 1974 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Analysis by Digestion and Colorimetric Assay of Total Nitrogen in Plant Tissues High in Nitrate1

D. A. Cataldo, L. E. Schrader and V. L. Youngs2

A method is proposed for analysis of total nitrogen in plant tissues enabling predigestion (to reduce nitrate to ammonium) and digestion to be performed in a Folin-Wu tube. Following digestion, a sensitive colorimetric assay for ammonium is used to quantitate N-content in an aliquot of the digest. The proposed method has several advantages: 1) up to 110 tissue samples can be predigested and digested in one batch; 2) small tissue samples (5 to 100 mg) can be accommodated; 3) the colorimetric assay for ammonia is very sensitive; 4) tissues can be predigested to recover all nitrate in the samples; and 5) aliquot size from the diluted digest can be varied to effectively detect as little as 1 µg of N.

Key Words: Micro-Kjeldahl

1 Cooperative investigation, ARS, USDA, and the Agricultural Experiment Station, University of Wisconsin, Madison. Research supported by the College of Agricultural and Life Sciences, University of Wisconsin-Madison, by USDA Cooperative Agreement 12-14-100-10,888, and by an award from the UW Graduate School Research Committee. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable.

2 Former Post Doctoral Fellow, Department of Agronomy, University of Wisconsin, Madison, WI 53706 (present address: Battelle, Pacific Northwest Laboratories, Ecosystems Department, Richland, WA 99352); Associate Professor, Department of Agronomy, University of Wisconsin, Madison, WI 53706; and Research Chemist, Oat Quality Laboratory, USDA and Associate Professor, Department of Agronomy, University of Wisconsin, Madison, WI 53706.

Received for publication April 5, 1974.


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