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Mitochondrial DNA from Maize Hybrids with Normal and Texas Cytoplasms¹

Mitochondrial DNA (mtDNA) was isolated from two hybrids of maize (Zea mays L.), NC232 x T204N (normal cytoplasm) and NC232 x T204 cms T (Texas cytoplasm). No difference was found in the buoyant densities of mtDNA isolated from the two hybrids. The mtDNA had a buoyant density of 1.706 g/cm³ in CsCl and a molar % guanine plus cytosine of 46.9. Therefore, the cytoplasmic dissimilarities with respect to sterility and disease response between the normal and the Texas cytoplasm cannot be accounted for by detectable alterations in the base composition of mtDNA.

Key Words: Zea mays L. • Organelle DNA • Buoyant density

² Graduate Student and Professor, respectively, Department of Genetics, North Carolina State University, Raleigh, NC 27607.

Received for publication June 17, 1974.

This article has been cited by other articles:

				C. S. LEVINGS III and D. R. PRING Restriction Endonuclease Analysis of Mitochondrial DNA from Normal and Texas Cytoplasmic Male-Sterile Maize Science, July 9, 1976; 193(4248): 158 - 160. [Abstract] [PDF]