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Simultaneous addition of lysine and threonine inhibits growth in maize (Zea mays L.) callus cultures and embryo cultures from several cereals. Methionine or homoserine reverses lysine-threonine inhibition. Isolation of feedback inhibition resistance mutants is proposed for several cereals by selecting callus or seedlings which grow in the presence of lysine-threonine. Resulting mutants may be overproducers of lysine, threonine, or methionine.
Key Words: Protein quality Tissue culture Embryo culture
2 Assistant and Associate Professor, respectively
Received for publication August 1, 1974.
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