Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 14:827-830 (1974)
© 1974 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Potential Selection System for Mutants with Increased Lysine, Threonine, and Methionine in Cereal Crops1

C. E. Green and R. L. Phillips2

Simultaneous addition of lysine and threonine inhibits growth in maize (Zea mays L.) callus cultures and embryo cultures from several cereals. Methionine or homoserine reverses lysine-threonine inhibition. Isolation of feedback inhibition resistance mutants is proposed for several cereals by selecting callus or seedlings which grow in the presence of lysine-threonine. Resulting mutants may be overproducers of lysine, threonine, or methionine.

Key Words: Protein quality • Tissue culture • Embryo culture


1 Contribution from Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55101. Paper no. 8616, Scientific Journal Series, Minnesota Agricultural Experiment Station.

2 Assistant and Associate Professor, respectively

Received for publication August 1, 1974.





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Copyright © 1974 by the Crop Science Society of America.