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Published in Crop Sci 14:770-771 (1974)
© 1974 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Rapid Procedure for Isolation of Polyhaploids in Bromegrass1

Geok Yong Tan and G.M. Dunn2

Cotyledons of Bromus inertnis Leyss. were fixed and decolorized in Carnoy's solution for 24 hours, immersed in 70% alcohol, and softened in lactic acid at 60 C for 15 min. The whole leaf was then mounted in lactic acid. Mean stomatal lengths of 41.8 and 43.4 µ were obtained for adaxial and abaxial surfaces of cotyledons of tetraploid seedlings compared to 62.3 and 65.1 µ for octoploid seedlings. This simple technique provides a rapid and economical method of screening for polyhaploids in populations of octoploid Bromus inermis.

Key Words: Stomatal length • Cotyledon • Octoploid • Seedling • Bromus inermis Leyss

1 Published with the approval of the Director of the New Hampshire Agricultural Experiment Station as Scientific Contribution No. 722 and as contribution from the NE-74 Regional Forage Technical Committee.

2 Plant Breeder, Chemara Research Station, Seremban, Malaysia; and Professor of Plant Science and Genetics, Plant Science Department, University of New Hampshire, Durham, NH 03824.

Received for publication April 22, 1974.




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