Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 14:676-678 (1974)
© 1974 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Influence of Maize Endosperm Mutants on Ground Kernel In Vitro Dry Matter Disappearance1

D. L. Garwood, J. S. Shenk and R. F. Barnes2

Ground kernels of the W64A maize (Zea mays L.) in. bred and 5 single and 10 double endosperm mutants of maize converted to W64A were evaluated using a twostage in vitro rumen fermentation system with and without urea added to the digestion buffer. In vitro dry matter disappearance (IVDMD) values were not correlated with protein content. Urea addition raised IVDMD values to the same level for most genotypes. Four genotypes were investigated using only first-stage digestion for 3, 6, 12, 24, and 48 hours. Maximum differences were observed after 12 and 24 hours of digestion. At these times, opaque-2 was most degraded followed by waxy, normal, and amylose-extender. The superior rate of opaque-2 digestion compared to waxy could not be explained by differences in particle sizes nor by known rates of isolated starch granule digestion. We suggest that incubation times used for forage IVDMD studies utilizing the basic procedure of Tilley and Terry be shortened when comparing ground grain samples.

Key Words: Opaque-2 maize • Waxy maize • Digestibility • Zea mays L.

1 Cooperative investigation of The Pennsylvania Agricultural Experiment Station and the U.S. Regional Pasture Research Laboratory, ARS, USDA, University Park, PA 16802. Authorized for publication on May 30, 1973 as Paper No. 4464 in the Journal Series of The Pennsylvania Agricultural Experiment Station. Contribution No. 305 of the U.S. Regional Pasture Research Laboratory.

2 Assistant Professor of Plant Breeding, Department of Horticulture, and Associate Professor of Plant Breeding, Department of Agronomy, The Pennsylvania State University; and Research Agronomist and Director, U.S. Regional Pasture Research Laboratory, ARS, USDA.

Received for publication June 11, 1973.




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Environmental Quality		 The Plant Genome
Copyright © 1974 by the Crop Science Society of America.