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Rapid Procedure for Estimating Cyclic Hydroxamate (DIMBOA) Concentration in Maize¹

A rapid procedure was developed for estimating the concentration of a cyclic hydroxamic acid 2,4-dihydroxy- 7-methoxy-l,4-benzoxazin-3-one (DIMBOA) for potential use in a maize (Zea mays L.) breeding program. Samples (0.2 to 0.5 g stem tissue) were collected from seedlings at 36 cm to 41 cm extended leaf height. The rapid procedure, essentially a simplification of the colorimetric procedure of Hamilton, was significantly correlated with the latter (r = +0.82) based on the analysis of 13 inbred lines of maize.

Key Words: Northern corn leaf blight • Cyclic hydroxamate • Biological resistance

² Graduate Research Assistant and Professors of Plant Science, Plant Science Department, University of New Hampshire, Durham, NH 03824. The authors express their appreciation to Drs. A. R. Hallauer, Ames, Iowa, and R. H. Hamilton, The Pennsylvania State University, for the inbred lines.

Received for publication February 28, 1974.