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Published in Crop Sci 14:441-442 (1974)
© 1974 Crop Science Society of America
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Method of Determining Genome Locations of Single Genes in Wheat1

O. G. Merkle, F. J. Gough and J. A. Green2

Wheat (Triticum aestivum L.) seedling reactions to stem rust (Puccinia graminis Pets. f. sp. tritici Erlks. and E. Henn.) were determined for F2 populations from selfed pentaploids from crosses between a susceptible tetraploid (AABB) and two resistant hexaploid lines (AABBDD). One of the hexaploid lines had Sr6 located on chromosome 2D and the other line had Srl6 located on chromosome 2B. A good fit to 3:1 ratio occurred when the resistance gene was located on chromosome 2B. The F2 segregation differed from the single gene Mendelian ratio when the resistance gene was located on chromosome 2D. The test demonstrated the feasibility of assigning resistance genes to the proper genome in single-gene hexaplold lines by crossing to appropriate tetraploid tester lines and testing the F2 populations.

Key Words: Triticum aestivum L. • Pentaploids • Genome analysis • Genetics

1 Contribution from the Agricultural Research Service, USDA and the Texas Agricultural Experiment Station. Approved for publication as Technical Article No. 10712 by the Director of the Texas Agricultural Experiment Station, College Station, TX 77843.

2 Agronomist, Plant Pathologist, and Technician, respectively, ARS, USDA, College Station, TX 77843.

Received for publication September 24, 1973.




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