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The data from four F2 progenies from a cross of a normal endosperm, multiple aleurone maize (Zea mays L.) line with an o2/o2 single aleurone line were heterogeneous but do not disprove the hypothesis that the multiple aleurone phenotype is conditioned by a single dominant factor. This factor segregated independently of opaque-2. In non-opaque seeds, the effect of multiple aleurone layers on protein content of the endosperm or content of any amino acid was negligible. In o2/o2/o2 endosperms, the presence of multiple aleurone layers as compared to single aleurone layers increased slightly the content of all the amino acids that are increased in o2/o2/o2 endosperms as compared with O2/—/— endosperms. Most of the amino acids that were lower in o2/o2/o2 endosperms relative to O2/—/— endosperms were decreased further by the presence of multiple aleurone layers. We conclude that the multiple aleurone layer factor should be useful in breeding programs designed to utilize the opaque-2 mutant.
Key Words: Lysine Opaque-2
2 Professor of Genetics and Project Specialist, Department of Genetics, University of Wisconsin, Madison, WI 53706.
Received for publication June 16, 1973.
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