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Variation in Protein Percentage in Different Portions of Peanut Cotyledons¹

Six varieties of peanuts (Arachis hypogaea L.) were studied for variation in percent protein by macro-Kjeldahl analysis of small sections from four consecutive segments (I, II, III, and IV) of the two cotyledons, with section IV at the embryonic end of the individual seeds. The distribution of percent protein in the cotyledon was significantly different within each variety. Four small- and medium-seeded varieties showed slightly higher protein in both end sections (I and IV) of the seeds than in the middle sections (II and III). High correlation coefficients were obtained between the protein percent of each section and that of the whole cotyledons except for section IV of the ‘Argentine’ variety. However, the combined sample from sections I and II produced less variability and gave an accurate estimate of percent protein for the whole cotyledons. All three seed parts of the six peanut varieties showed a significant difference in percent protein. The embryonic axis and the cotyledon had about the same amount of protein, but each had a much higher protein percent than did the seed coat.

Key Words: Arahis hypogaea L. • Seed coat • Embronic axis

² Postdoctoral Fellow and Assistant Professor, respectively.

Received for publication June 16, 1973.