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Published in Crop Sci 14:120-122 (1974)
© 1974 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Olfactory Screening of Alfalfa Clones for Uniform Honey Bee Selection1

G. M. Loper, Gordon D. Waller and Richard L. Berdel2

An olfactory bioassay procedure is described, and results from testing 28 alfalfa (Medicago sativa L.) clones for honey bee (Apis mellifera L.) olfactory compatibility are reported. Honey bee selection appeared to be based on quantity and quality of flower volatiles. When the honey bees were conditioned to one clone ("normal" alfalfa flower volatiles), bee selection indicated that alfalfa clones could be classified into three categories: compatible, neutral, and incompatible. The difference between compatible and neutral seemed to be based on lower quantities of volatiles produced by the neutral group; however, the one incompatible clone was discriminated against on the basis of olfactory quality. This bioassay procedure could be used as a tool for alfalfa breeders to select parent clones that are olfactorily compatible.

Key Words: Flower volatiles • Pollination • Seed production • Terpenes • Olfacion vs GC • Aroma bioassay • Bee behavior

1 Contribution from the Agricultural Research Service, USDA, and the Arizona Agricultural Experiment Station. Journal Article No. 2145.

2 Plant Physiologist, Research Entomologist, and Research Technician, respectively. ARS, USDA, 2000 E. Allen Road, Tucson, AZ 85719.

Received for publication August 31, 1973.


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L. Pecetti and A. Tava
Effect of Flower Color and Sampling Time on Volatile Emanation in Alfalfa Flowers
Crop Sci., January 1, 2000; 40(1): 126 - 130.
[Abstract] [Full Text]


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