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Liquid Water Content of Cell Walls in Frozen Tissues Evaluated by Electrophoresis of Indicators¹

The content of liquid water at equilibrium with ice in a frozen cellulose model was evaluated by electrophoretic mobility of indicators, and a method of translating relative moisture data of the type previously obtained from plant tissues to absolute values is presented. Absolute water distribution data are required for coordination with calorimetric data, so that stress energies can be calculated and plant systems capable of modifying stress can be identified.

Key Words: Water • Freezing stress

² Plant Physiologist, North Central Region, ARS, USDA, and Post Doctorate Researcher, Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48823.

Received for publication May 9, 1973.