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Vigor Determination in Soybean Seed by Multiple Criteria¹

A multiple criteria approach is presented by which vigor was evaluated in 16 lots of soybean (Glycine max L., cv. ‘Lee-68’) seed by determining O₂ uptake, CO₂ production, uptake of labeled glucose or leucine, conversion of isotopes into ¹⁴CO₂ and polysaccharides or proteins, and leaching of metabolites through membranes of excised embryonic axes after 5 hours of imbibition. The concept upon which this approach is based is that excised embryonic axes from vigorous seed lots take up more sugars and amino acids from imbibing media, incorporate these metabolites faster into polysaccharides and proteins, and permit less leaching of nonused metabolites into surrounding aqueous media than axes from less vigorous seed lots. These measurements focus on the biosynthetic capacity of the axis and the integrity of its cellular membranes.

Key Words: Leguminaceae • Soybean • Embryonic axes • Respiration • Germination • Glucose metabolism • Amino acid metabolism • Leaching • Membrane permeability

² Plant Physiologists, Post Harvest Plant Physiology Laboratory, ARS, USDA, Beltsville, Md. 20705.

Received for publication April 7, 1973.