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Pollen Storage of Certain Agronomic Species in Liquid Air¹

Viability of liquid air-stored pollen from alfalfa (Medicago sativa L.), soybeans (Glycine max L. Merr.), cotton (Gossypium hirsutum L.), corn (Zea mays L.), oats Avena sativa L.), rye (Secale cereale L.), sorghum (Sorghum bicolor (L.) Moench), and wheat (Triticum aestivum (L.) em Thell) was measured by in vitro germination, staining with tetrazolium bromide, and pollination. Some stored pollen of alfalfa, soybeans, cotton, and rye germinated in vitro. Stored pollen of all eight species exhibited variable levels of viability when stained. Successful pollinations of soybeans and rye were obtained with stored pollen. Results of germinating in vitro and staining of fresh pollen compared favorably. The staining method was simpler and gave more consistent results than in vitro germination. In addition, staining can be used for species for which suitable artificial germination media have not been developed.

Key Words: Alfalfa • Corn • Cotton • Oats • Rye • Sorghum • Wheat • in vitro Germination • Staining

² Assistant Professor of Agronomy, former F.A.O. graduate student (presently staff member Bangkhen Agricultural Experiment Station, Bangkok, Thailand), and Professor of Plant Pathology, respectively, University of Arkansas, Fayetteville, Arkansas 72701.

Received for publication March 10, 1973.