Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 13:491-493 (1973)
© 1973 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Inbred and Hybrid Effects on Establishment of in Vitro Cultures of Zea Mays L. Endosperm1

J. C. Shannon and J. W. Batey2

Zea mays L. endosperm explants from 23 starchy inbred and hybrid lines and several endosperm mutants were tested for their ability to grow in vitro. Explants from two starchy inbreds, two hybrids, and the mutants amylose-extender, sugary-1, and shrunken-2 grew actively and have been maintained in culture for over 2 years. Other lines produced limited growth or no growth following the first transfer. These data demonstrate the importance of testing several different inbreds or hybrids when attempting to establish in vitro cultures of corn endosperm.

Key Words: Tissue culture • Endosperm mutants

1 Authorized for publication on January 19, 1973, No. 4377 in the Journal Series of the Pennsylvania Agricultural Experiment Station. Partial support of this work was provided by USDA Cooperative Agreement No. 12-14-100-9066 (34) to the Indiana Agr. Exp. Sta.

2 Associate Professor, Department of Horticulture, Pennsylvania State University, University Park, Pa. 16802; and Assistant Professor, Department of Biology, Seton Hall University, South Orange, N.J. 07079.

Received for publication February 1, 1973.




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Environmental Quality		 The Plant Genome
Copyright © 1973 by the Crop Science Society of America.