Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 13:462-463 (1973)
© 1973 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Selection of High Lysine Corn Using a Scanning Electron Microscope1

C. E. Wassom and R. C. Hoseney2

Several inbred sources of corn (Zea mays L.), including high-lysine modified opaque-2 lines with hard, translucent endosperm and normal hard, translucent endosperm phenotypes were examined with a scanning electron microscope. Zein bodies were observed in the protein matrix of the normal corn phenotype. Placing samples in 70% ethanol and observing voids produced confirmed that the bodies contained zein. Samples from modified opaque2 lines known to be high in lysine did not show elaborate patterns of voids in the rotein matrix after ethanol treatment. It was conclufed that a scanning electron microscope is an excellent tool to identify endosperms with low zein and potentially more lysine.

Key Words: Maize • Zein • Protein

1 Contribution No. 1315. Department of Agronomy, No. 822, Department of Grain Science and Industry, Kansas Agricultural Experiment Station. Research supported in part by NIH Biomedical Sciences Support Grant RR-07036 and NSF Equipment Grant GBO32296. Kansas Agricultural Experiment Station.

2 Associate Professor of Agronomy and Associate Professor of Grain Science and Industry, Kansas State University, Manhattan. Kansas 66506.

Received for publication February 7, 1973.




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Copyright © 1973 by the Crop Science Society of America.