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A basic color factor gene C1 in diploid alfalfa (Medicago saliva L.) (S-2128) was found to be nonallelic with the basic color factor gene C2 in tetraploid alfalfa. The tetraploid recessive c2 was transferred down to diploid level by haploidy, and the diploid recessive C1 was transferred up to the tetraploid via 2n gametes. Both genes produced monohybrid segregation ratios of about 3:1 at the diploid level and tetrasomic ratios of about 35:1 in the tetraploid. The dihybrid F2 segregation of both genes at the diploid level was about 9:7, indicating recessive epistasis. The homozygous recessive condition of either c1 or c2 produces a white flowered, white seeded phenotype. Additional research is needed to determine the independence or linkage of the two genes. The genetic stocks were made available for this and other research.
Key Words: Medicago sativa Genetics Chromosome Haploid Gamete
2 Associate Professor of Agronomy.
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