Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 13:389-391 (1973)
© 1973 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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A Root Tip Squash Technique for Soybean Chromosomes1

R. G. Palmer and Hollys Heer2

A procedure is described for consistently obtaining adequatenumbers of well-spread metaphase cells from soybean [Glycine max (L.) Merr.] root tips. The salient features are: (i) seeds germinated at alternating temperatures of 30 C for 18 hours and 22 C for 6 hours until roots are 7 to 10 cm long; ii) pretreatment of root tips in saturated paradichlorobenzene at 15 C for 1 1/2 to 2 hours; and iii) fixation in 3:1 for 48 hours at 37 C before hydrolysis and staining in leuco-basic fuchsin. Tips were incubated in pectinase for 1 1/2 hours at 30 C, and the cells were flattened with a pellet press.

Key Words: Glycine max (L.) Merr. • Mitosis

1 Joint contribution of the Agricultural Research Service, USDA, and Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project 1179, Journal Paper No. J-7373. Mention of a trademark or proprietary product does not constitute a guarantee or warranty by the USDA or Iowa State University and does not imply approval to the exclusion of other products that also may be suitable.

2 Research Geneticist, ARS, USDA, and Technician, Agronomy Department, Iowa State University, Ames, Iowa 50010.

Received for publication October 16, 1972.




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