Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 13:332-334 (1973)
© 1973 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Relationship of Stomatal Length and Frequency and Pollen-Grain Diameter to Ploidy Level in Bromus inermis Leyss1

Geok-Yong Tan and G. M. Dunn2

Stomatal length and frequency and pollen grain diameter were examined as indirect methods for identification of 4x, 6x and 8x ploidy levels in Bromus inermis. The study was conducted in the greenhouse on seedlings and mature plants. Mean stomatal lengths were 40.4, 43.0, 53.1, and 66.6 µ for mature 4X (clonal), 4X (selfing), and 8X plants, respectively. Coefficients of variation among observations within each ploidy level were relatively low. Stomatal frequency decreased with increasing ploidy while pollen-grain diameter increased, but considerable overlapping occurred among ploidies for both characters. Greater ploidy differences and smaller standard deviations were associated with stomatal length rather than with stomatal frequency and pollen-grain diameter. Therefore, the former is recommended as a rapid method for identification of ploidy level.

Key Words: Tetraploid • Hexaploid • Octoploid • Grasses • Polyploidy

1 Published with the approval of the Director of the New Hampshire Agricultural Experiment Station as Scientific ContributionNo. 644 and as a contribution from the NE-74 Regional Forage Technical Committee. Part of a thesis submitted by the senior author in partial fulfillment for the M,S. degree.

2 Graduate Research Assistant and Professor of Plant Science and Genetics, Plant Science Department, University of New Hampshire.

Received for publication December 4, 1972.




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