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The tetraploid tissue of American Pima cotton (Gossypium barbadense L.) plants chimeral for tetraploid and haploid tissues was found to be of hybrid origin. We crossed 57-4, a doubled haploid that is semigametic, as female, with a multiple-marked stock homozygous for glandless and heterozygous for red plant color. A number of the plants from this cross were chimeral for glanded or glandless tissue, red or green tissue, and tetraploid or haploid tissue. The combinations of tissues in tri-chimeral plants were such that polyspermy appeared to be involved in the development of their tetraploid tissue. We theorized that a sperm nucleus divided in the egg cell before fertilization. The semigametic tendency of the female parent may be associated with the stimulation of divisions of the sperm nucleus.
Key Words: Amphidiploid Chimeral Gossyplum barbadense L. Haploid Polyspermy Semigamy Tetraploid
2 Research Geneticist and Research Agronomist, ARS, USDA, University of Arizona Cotton Research Center, Phoenix, Ariz. 85040.
Received for publication August 16, 1972.
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