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Published in Crop Sci 12:708-709 (1972)
© 1972 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Cyanoglucoside and Hydrolyzing Enzyme in Species Related to Trifolium repens1

Pryce B. Gibson, O. W. Barnett and J. T. Gillingham2

Trifolium repens L. and 16 species closely related to T. repens were tested qualitatively for the presence of cyanoglucoside and the enzyme that releases HCN by hydrolysis of glucoside in damaged leaves. In contrast to T. repens, none of these species contained enzyme in the absence of glucoside. Four species were comprised entirely of plants showing both constituents, one of plants showing glucoside only, and eight of plants showing neither constituent. The remaining three species were alike in that some plants contained glucoside and some did not.

Key Words: Cyanogenesis • Prussic acid • Phytochemistry • Trifolium speciation

1 Cooperative investigations of the Plant Science Research Division, ARS, USDA, and the South Carolina Agricultural Experiment Station, Clemson, S.C. Published with the approval of the Director of the South Carolina Agricultural Experiment Station as Technical Paper No. 995.

2 Research Agronomist, PSRD, ARS, USDA; Assistant Professor, Department of Plant Pathology and Physiology, and Associate Chemist, Chemical Services (Agr.) South Carolina Agricultural Experiment Station, Clemson, S. C. 29631.

Received for publication March 6, 1972.




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