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Studies on the Activity of C.P.41845 in Early-Juvenile Sugarcane

A plantlet method for evaluating sugarcane (Saccharum spp.) ripeners was developed using leaf-blade and excisedstorage tissues for sucrose and enzyme analyses. Activity parameters were selected on the basis of chemical sensitivity in adult cane and were tested in conjunction with applied C.P.41845 as the standard ripener. Early-juvenile plants propagated in quartz sand with controlled water and nutrient supplies were sprayed about 4 weeks after germination. Aqueous sugar and protein extracts were prepared with lyophilized leaf and immature storage tissues harvested at 5 or 10 day intervals after treatment. Sucrose, water-soluble protein, acid invertase, and beta-amylase values deviated significantly from the control after 5 days and the trends usually persisted up to 15 or 25 days. Divergent growth regimes produced with variable nitrate did not alter the chemical action or parameter sensitivity. Results were consistent with the view that chemicals having authentic ripening potential in adult cane will reflect this potential during the early-juvenile stage when suitable parameters are measured.

Key Words: Saccharum officinarum L. • Chemical ripening • Chemical screening • Plantlet physiology

Received for publication February 14, 1972.