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Effects of Nutrient-Media Composition on Growth of Seedlings from Intact Seeds and Excised Embryos of Maize (Zea Mays L.)¹

Basal and complex agar-sucrose nutrient media were compared for effects on growth of light-grown seedlings from mature intact seeds and excised embryos of maize (Zea mays L.). Effects of auxin, 2, 4-dichlorophenoxyacetic acid, also were studied.

Complex medium increased root growth of seedlings from intact seeds but did not affect root growth of seedlings from excised embryos. There were no nutrient media effects on shoot growth. No absolute requirement by cultured maize embryos for externally supplied auxin was demonstrated. In the basal medium, auxin inhibited root growth.

Key Words: Auxin • 2, 4-D • Sterile culture

² Postdoctoral Research Associate and Assistant Professor, Agronomy Department, Iowa State University, Ames. Iowa 50010. (Present address of senior author, Merck, Sharp, and Dohme International, Rahway, New Jersey 07065).

Received for publication February 16, 1972.