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Stomate Density and Size as Related to Ozone-Induced Weather Fleck in Tobacco¹

A study was conducted to investigate the relationship between stomate density and size on reaction of tobacco (Nicotiana tabacum L.) to elevated concentrations of ozone. The resistant varieties ‘Florida 20’ and ‘C-80’ averaged 25.4 stomates per mm² on the adaxial leaf surface, while the susceptible varieties ‘2612-2,’ ‘2612-3,’ and ‘Bel W-3’ averaged 37.4. This decrease in stomate density by one-third in resistant varieties is thought to be associated with resistance by reducing the amount of ozone-rich air which can enter the leaf. No relationship was found between stomatal size and plant reaction to ozone.

Key Words: Nicotiana tabacum • Cigar-wrapper • Varieties

² Professor (Agronomist), University of Florida, IFAS, Department of Agronomy, Gainesville, Florida 32601.

Received for publication February 4, 1972.

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