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Influence of Temperature at Various Growth Stages of Oat Cultivars. I. Utilization of Controlled Environment Chambers, Climatological Data and Developmental Growth Stages¹

An approach is discussed that permits an evaluation of genotype x temperature and growth stage x temperature interactions in oats (Avena sativa L.). Methods were developed that permitted the assay (a) of genetic differences for tolerance to temperature stress among four oat cultivars and (b) of reactions of four oat cultivars when stress temperatures were applied at several growth stages.

Controlled environment chambers were programmed to simulate climatological data for the Iowa oat-growing season of April through June. Three temperature levels (high, control, and low) were imposed at five growth stages (initiation and differentiation of tillers, differentiation of floral reproductive structures, elongation of shoot apex, anthesis, and grain filling) on four diverse oat cultivars.

Even though an experimental unit consisted of only one plant per 4-inch pot, the precision of experimentation, measured by coefficients of variation (CV), was quite acceptable. The CV's for seed yield varied from 8.2 to 9.6%, similar to oat yield trials in the field where the sample size is about 500 plants.

Key Words: Genotype x temperature interactions • Avena sativa L.

² Associate Professor of Agronomy and Genetics, Plant and Soil Science Department, Montana State University, Bozeman 59715 (formerly Research Associate at Iowa State University) and Professor of Plant Breeding, Iowa State University.

Received for publication November 12, 1971.