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Published in Crop Sci 12:253-254 (1972)
© 1972 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Two Methods of Tannin Analysis for Sorghum Bicolor (L.)Moenchgrain1

E. D. Maxson and L. W. Rooney2

Urea extraction followed by reaction with ferric ammonium sulfate (FAS) and a modified vanillin-hydrochloric acid (MV.HC1) technique are two promising methods for analysis of polyphenolic compounds (tannins) sorghum [Sorghum bicolor (L.) Moench] grain. Both procedures are presented. Tannic acid values (FAS) ranged from 0.20 to 0.76 mg/100 mg and catechin equivalents (MV-HC1) ranged from 0.21 to 3.11 rag/100 mg. The MV-HCm1 ethod is rapid, but has a significant cultivar x day interaction. The FAS method is slower; but does not exhibit the interaction.

Key Words: Polyphenols • Cereals • Grain sorghum • Sorghum composition

1 ~Contribution from the Cereal Quality Laboratory, Texas Agricultural Experiment Station, Texas A&M University, College Station, Texas. Part of this manuscript is a portion of a thesis submitted by the senior aothor to the Texas A&M University graduate faculty in partial fulfilhnent of requirelnents for an M.S. degree in food technology.

2 Technician and Associate Professor, Cereals Laboratory, Soil and Crop Sciences Department, Texas A&M University, College Station, Texas.

Received for publication July 26, 1971.




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