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Stable Foam Volume and Pectin Methyl Esterase Activity of Forage Mixtures¹

Alfalfa (Medicago sativa L.), birdsfoot trefoil (Lotus corniculatus L.), crownvetch (Cornilla varia L.), sainfoin (Onobrychis viciaefolia Scop.), and Kentucky bluegrass (Poa pratensis L.) forage samples were tested individually and in two species component mixtures for stable foamproducing capacity and for pectin methyl esterase activity. Oven drying at 100 C reduced average foam volume by 18% of the wet sample mean. Species interacted in a nonadditive manner during all foam viscosity tests. The magnitude of the deviations from the averages of the component species responses was not as great as observed in an earlier investigation. Liquid viscosities of forage mixtures as expressed by their pectin methyl esterase activities were dependent solely upon the mean values of the component species tested alone. Results of the two types of empirical in vitro procedures were positively and significantly correlated, = .86.

Key Words: Alfalfa • Bloat • Crownvetch • Grass • Legume • Trefoil • Sainfoin

² Professor, Plant Science Department, South Dakota State University, Brookings, South Dakota 57006.

Received for publication May 27, 1971.