Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 11:935 (1971)
© 1971 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Low Temperature Maintains Virulence of Dry Colletotrichum Trifolii Inoculum1

S. A. Ostazeski, T. A. Campbell, T. E. Devine and C. H. Hanson2

Dry Colletotrichum trifolii inoculum from alfalfa (Medicago saliva L.) stored 10 months in a freezer at –20 C, or in a refrigerator at 7.5 C, was as virulent as freshly blended V-8 juice agar cultures. Dry inoculum stored at 21 C was nearly avirulent at the end of 4 months.

Key Words: Disease • Resistance • Anthracnose • (Medicago saliva L. • Pathogen • Breeding


1 Contribution from the Plant Science Research Division, Agricultural Research Service, US Department of Agriculture, Beltsville, Md. 20705.

2 Research Plant Pathologist, Agronomist (Research Assistant), Research Geneticist, and Research Agronomist, respectively. Plant Science Research Division, Agricultural Research Service, US Department of Agriculture, Beltsville, Md.

Received for publication March 10, 1971.





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