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Published in Crop Sci 11:816-818 (1971)
© 1971 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Influence of Iron Supply on Peroxidase Activity and Peroxidase Isozymes in Corn (Zea mays)1

R. L. Ulmer, F. A. Haskins and W. R. Akeson2

Plants of Oh43 x Wf9 corn (Zea mays L.), grown in sand culture system, were watered daily with nutrient solution containing 0.025, 1.25, or 2.5 ppm Fe. Plants also were grown in a soil-sand mixture. Leaf extracts were assayed quantitatively for peroxidase activity, and sampies of the extracts were subjected to gel dectrophoresis. A positive relationship was observed between Fe supply and proxidase activity. Numerous peroxidase isozymes were detected by gel electrophoresis. As judged by intensity of the hands, all isozymes appeared to be influenced by Fe supply.

1 Published with the approval of the Director as Paper No. 3072, Jonrnal Series, Nebraska Agr. Exp. Sta. Research reported was conducted under Projects 12–1 and 12–50 and was supported in part by a grant from the Rockefeller Foundation to the corn research program at: Nebraska. Data were taken from a thesis submitted by the senior author to the University of Nebraska in partial fulfillment of the requirements for the M.S. degree.

2 Graduate Assistant, Bert Rodgers Professor, and Assistant Professor, respectively, Department of Agronomy, University of Nebraska, Lincoln. Current address of Dr. Akeson: Great Western Sugar Co., Longmont, Colo.

Received for publication December 9, 1970.




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