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Variability Associated with Sampling of Rhizobium japonicum Populations in Soybeans¹

Five hundred nodules from the soybean [Glycine max (L.) Merrill] variety ‘Lee’ were serotyped each of 3 years. The Rhizobium japonicum [(Kirchner) Buchanan] strains in the nodules were classified into serogroups according to their serological reaction. Standard errors of the mean were computed for the predominant serogroups to determine the variability of the R. japonicum population within the field. R. japonicum variability was found to be greater within than between areas (blocks) in the field.

Key Words: Nitrogen fixation • Serology • Glycine max • Bacteria • Nodulation

² Plant Pathologist and Agronomist, respectively, Plant Science Research Division, Beltsville, Md., and Biometrician, Biometrical Services, Office of Administrator, Agricultural Research Service, US Department of Agriculture, Fort Collins, Colo. Current address of senior author: Department of Horticulture, Michigan State University, East Lansing, Mich. 48823.

Received for publication February 28, 1969.