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Formononetin Levels in Subterranean Clover (Trifolium subterraneum L.) as Affected by Phosphorus and Sulfur Nutrition¹

Effects of six rates of P and three of S on the level of the estrogenic isoflavone formononetin in ‘Dwalganup’ subterranean clover (Trifolium subterraneum; L.) were evaluated. With increasing rates of fertilizer P and S the concentration of formononetin in leaf blades decreased significantly (at the 1% level). The P x S interaction was not statistically significant. The decrease in formononetin concentration may have resulted from a "dilution effect" and/or a decrease in isoflavone synthesis.

Key Words: Estrogens • Plant estrogens • Isoflavones • Soil fertility

² Graduate student and Assistant Agronomist, respectively, Department of Agronomy and Range Science.

Received for publication March 27, 1971.