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Published in Crop Sci 11:580-582 (1971)
© 1971 Crop Science Society of America
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Leaf Characteristics of Four Flue-Cured Tobacco Varieties According to Stalk Positions. I. Phytosterols and Petroleum Ether Extracts1

Amber L. S. Cheng2, T. C. Tso2 and James F. Chaplin3

Phytosterol content and petroleum ether extracts varied in leaves from various stalk positions of three flue-cured tobacco (Nicotiana tabacum L.) varieties and one breeding line, ‘Coker 139,’ ‘SC 58,’ ‘NC 95,’ and LN 38. These varieties differ widely in alkaloid content. Petroleum ether extracts were not associated with alkaloid content. Phytosterol content of leaves of various stalk positions exhibited the same general distribution patterns among different varieties, while petroleum ether extracts exhibited the same general distribution patterns in three varieties but not in LN 38. Four phytosterol fractions, including cholesterol, campesterol, ß-sitosterol, and stigmasterol, were determined. Cholesterol and campesterol were fairly constant in leaves of various stalk positions; however, an increase of ß-sitosterol and a decrease of stigmasterol in leaves from bottom to top stalk positions was noted.

Key Words: Nicotiana tabacum L. • Cholesterol • Stigmasterol • Campesterol • ß-sitosterol


1 Contribution from the Plant Science Research Division, Agricultural Research Service, U. S. Department of Agriculture and North Carolina State University, Agricultural Experiment Station, Raleigh, N. S. Paper No. 3376 of the Journal Series of the North Carolina State University and Agricultural Experiment Station, Raleigh, N. C.

2 Research Chemist and Plant Physiologist, PSRD, ARS, U. S. Department of Agriculture, Beltsville, Md. 20705.

3 Research Agronomist, PSRD, ARS, U. S. Department of Agriculture, Oxford, N. C. 27565, and Professor of Crop Science, North Carolina State University, Raleigh.

Received for publication February 1, 1971.





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