HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Fromberg, H. K. Articles by Pollmer, W. G. Search for Related Content PubMed Articles by Fromberg, H. K. Articles by Pollmer, W. G. Agricola Articles by Fromberg, H. K. Articles by Pollmer, W. G.

A Rapid Method Based on Butanol Extraction for Selecting High-Lysine Maize (Zea mays L.)

Whole kernel samples from 48 selfed lines (S₂, S₃ of maize (Zea mays L.) were obtained from crosses involving normal and the mutant opaque-2. Kernels from these samples were classified as translucent, semitranslucent, and opaque in appearance. All were treated with watersaturated n-butanol at room temperature. The extraction resulted in losses of nitrogen ranging from 0 to 40%. The nitrogen compounds extracted were nearly free of lysine. The proportion of butanol-insoluble nitrogen to total nitrogen was closely correlated with lysine content of the protein, with an r value of .817. Similarly, the insoluble nitrogen was closely correlated (r = .847) with the lysine content on a whole-kernel basis. Since the method requires only the simple procedures of nitrogen determination and butanol extraction, it should be very suitable for screening a large number of maize samples for high lysine.

Key Words: Opaque-2 • Protein quality • Protein quantity

² Research Assistant, Plant Breeding II; Abteilungsvorsteher, Food Technology; Professor, Abteilungsvorsteher, Plant Breeding II, University of Hohenheim, POB 106, 7000 Stuttgart 70, Fed. Rep. of Germany.

Received for publication January 12, 1971.