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Interchanges that produce haplo-viable deficiency-duplications have several possible uses. This paper reviews the behavior of these interchanges and relates that behavior to methods of determining linkage between a gene and an interchange breakpoint and their relative order merely by observing the segregation ratios of the unplaced gene in backcross or F2 populations. The method has the advantage that no additional marker gene or pollen classification is needed. Documented and predicted haploviable deficiency-duplication generating interchanges of maize (Zea mays L.) are listed. A linkage-detection tester set has been selected marking 19 of the 20 chromosome arms. Data are reported to substantiate the feasibility of the method. Additional uses of these interchanges include their employment in (1) inheritance studies on quantitative traits, (2) obtaining genetic markers for chromosome-end segments, and (3) the production of duplications for terminal chromosome segments.
Key Words: Reciprocal Translocations Linkage detection Chromosome markers Cytogenetics Maize
2 Assistant Professor and Professor, University of Minnesota, St. Paul, and Assistant Professor, University of Illinois, Urbana.
Received for publication January 24, 1968.
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