Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 11:387-390 (1971)
© 1971 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Analysis of Two Enzyme Proteins in Some Gcnotypes of Corn (Zea mays L.)1

N. K. Chatterjee and C. O. Gardner2

Two enzyme proteins [alcohol dehydrogenase (ADH) and esterase] were analyzed electrophoretically; where possible, kinetic measurements for ADH were made in two exotic varieties of corn (Zea mays L.), some elite inbred lines and their hybrids, and two mutant lines developed by ethyl methane-sulfonate (EMS) and thermal neutron treatments. Starch gel electrophoresis in a discontinuous buffer system for the two enzymes indicated their existence in multiple forms (isozymes) in the genotypes studied. All of the inbreds, except one, had two anode-migrating isozymes of ADH, one of which was fast and the other slow moving. Of two mutants (EMS- and thermal neutron-induced) from one inbred, only one (EMS) showed an isozyme pattern different from the original line. All single and double crosses showed the presence of hybrid enzyme in addition to the parental enzymes. Estimated specific activity values of ADH were lower in all the hybrids, except one, in comparison with the corresponding mid-parent values. Km's for ethanol incrude enzyme preparations of the hybrids were lower than those of the mid-parents of the hybrids. Km's of the two mutants were higher than that of the control.

Three of five anode-migrating isozymes of esterase were found in the different genotypes. The EMS mutant showed an isozyme pattern different from the control. No hybrid enzyme for esterase was found in the hybrids. Possible use of isozyme studies in populations and sets of lines derived from them, and relating such studies to grain yield, are discussed.

Key Words: Isozymes • Esterase • Alcohol dehydrogenase

1 Contribution from the Department of Agronomy, University of Nebraska. Published with the approval of the Director as Paper No. 2979. Journal Series, Nebraska Agricultural Experiment Station, Lincoln Nebraska, 68503 and as Atomic Energy Commission Technical Information Document C00-1512-19. Research reported was conducted under project 12-49.

2 Research Associate, Division of Biochemistry, Department of Chemistry, and Meyer Katzman Professor of Agronomy, University of Nebraska.

Received for publication October 27, 1970.




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