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Oxidation and Phosphorylation By Mitochondria Isolated From Iron-sufficient and Iron-deficient Corn Roots

Active mitochondria were successfully isolated from roots of Fe-deficient and Fe-sufficient corn (Zea mays L.) plants. Citrate, DL-isocitrate, -ketoglutarate, succinate, L-malate, and NADH were oxidized by the mitochondria from either kind of corn plant. Fe-deficient root mitochondria had decreased O₂ uptake and oxidative phosphorylation as compared to those of Fe-sufficient root mitochondria. Furthermore, mitochondrla from Fe-deficient corn roots had a low respiratory control ratio and the O₂ uptake had little response to the addition of ADP. Measurements of NADH oxidation by a spectrophotometric method showed that Fe-deficient root mitochondria reduced the affinity between substrate and the nonheme Fe enzyme (NADH dehydrogenase).

Key Words: Respiratory control • Krebs' cycle substrates

Received for publication June 25, 1970.