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Published in Crop Sci 11:265-267 (1971)
© 1971 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Alpha-amylase Activity and Carbohydrate Content as Related to Kernel Development in Triticale1

A. J. Klassen, R. D. Hill and E. N. Larter2

Eight lines of triticale (Triticale hexaploide) selected for variation in degree of kernel shrivelling were analyzed during development and at maturity for {alpha}-amylase activity, reducing sugars, and starch content. Alpha-amylase activity varied widely among the triticale lines and was inversely correlated with grain density, the index of shrivelling. Differences in {alpha}-amylase activity in turn were reflected in the reducing sugar values. Starch content of mature grain ranged from 49.1 to 57.1% and was significantly correlated with grain density. Alpha-amylase developmental patterns were similar at early stages for 6A320, a line with better kernel type, and 6A190, a line with poor kernel development. However, at about 55% moisture, {alpha}-amylase activity in 6A190 began to increase rapidly as compared to 6A320. Reducing sugar patterns were similar to those for {alpha}-amylase. Patterns of starch development indicated a premature termination of starch deposition in triticale lines with poor kernel characteristics.

Key Words: Wheat-rye hybrids • Kernel shrivellings • Grain density • Starch • Reducing sugars


1 Contribution No. 248 of the Department of Plant Science, University of Manitoba, Winnipeg 19, Manitoba, Canada.

2 Graduate Student, Associate Professor, and Professor, Department of Plant Science.

Received for publication September 12, 1970.





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