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Soaking Injury in Lima Beans¹

We investigated water uptake and ribonudease development in excised embryonic axes of lima beans (Phaseolus lunatus L. cv. ‘Thorogreen’) during the period 0 to 18 hr after the start of imbibition. The impact of temperature, initial axis moisture, and submergence on these processes was studied. In general, ribonuclease development paralleled growth. However, under stress conditions enzyme formation was reduced to a greater degree than was growth. Submergence during the first 10 min of imbibition appeared tn condition the axes to reduced growth and ribonuclease development at a later period. Submergence during the growth period also had an adverse effect on these processes.

Key Words: Imbibition • Ribonuclease • Germination • Embryonic Axes • Phaseolus

² Plant Physiologists, Crops Research Division, ARS, USDA, National Seed Storage Laboratory, Fort Collins, Colorado 80521.

Received for publication July 20, 1970.