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Methods for Evaluation of Soybean Hypocotyl Length¹

We compared the effectiveness and efficiency of sand and paper-towel methods for the evaluation of hypocotyl length of soybean (Glycine max (L.) Merrill) genotypes at 25C. Seeds planted in sand at 10 cm in containers covered with plastic bags had hypocotyl lengths that were reproducible and consistent, Sand methods made individual hypocotyl measurements difficult. When the seeds were planted on standard germination towels, the results equaled those in the 10-cm sand if moisture was maintained at an adequate level. To obtain an adequate moisture level, it was necessary to use a 30- x 30-cm towel, and the containers had to be covered. Orientation of the seed with the mitropyle up on the paper towel prevented twisted hypocotyl~ which were difficult to measure. Seed treatment with a fungicide had "no influence on hyocotyl length, but may be of value with diseased seed lots.

Key Words: Glycine max (L.) Merrill • Seedling emergence • Sand method • Paper towel method

² Assistant Professor of Botany and Associate Professor of Agronomy and Collaborator, Crops Research Division, Agricultural Service, U.S. Department of Agriculture, Iowa State University, respectively.

Received for publication August 24, 1970.