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Published in Crop Sci 10:578-581 (1970)
© 1970 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Quantification of Hydrocyanic Acid in Green Forage1

J. C. Burns, L. H. Smith, W. J. Moline, W. F. Wedin, C. H. Noller and C. L. Rhykerd2

A procedure is presented for the quantification of potential hydrocyanic acid (HCN) released from green tissue. This procedure is suggested in preference to qualitative procedures because resulting data can be compared among both laboratories and regions. In addition, results from qualitative procedures frequently appear incorrectly on a quantitative basis.

The procedure was evaluated for its precision and recovery. An experiment was conducted to examine method (s) of sample preservation and period (s) of sample holding and incubation prior to analysis.

Forage sealed in a polyethylene bag and immediately frozen analyzed similarly in HCN to samples stored in the dark at 26 C. Once preserved, samples were held for 6 hr prior to analysis without significant reduction in HCN levels; however, a slight reduction was noted after 3 hr.

Maximum release of HCN was obtained after samples had been incubated in ethanol for 24 hr with no significant change through 96 hr.

Key Words: Sample preservation • Holding time • Incubation time • Plant tissue analysis

1 Paper number 3103 of the Journal Series of the North Carolina State University Agricultural Experiment Station, Raleigh, N. C. 27607, in cooperation with the Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture.

2 Plant Physiologist, Crops Research Division, ARS, USDA; Professor of Agronomy and Plant Genetics, University of Minnesota; Associate Professor of Agronomy, University of Nebraska; Professor of Agronomy, Iowa State University; Professor of Animal Sciences and Professor of Agronomy, Purdue University.

Received for publication March 16, 1970.




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