Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 10:503-506 (1970)
© 1970 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Uterotropic Activity and Heritability of Biochanin A and Formononetin in Trifolium medium L.1

L. M. Gourley, W. F. Keim and Martin Stob2

The four isoflavones, genistein, biochanin A, formononetin, and daidzein, were identified in zigzag clover, Trifolium medium L. Biochanin A and formononetin were the predominant isoflavones; genisteln and daidzein were present only in trace amounts. The isoflavones were separated by thin-layer chromatography and quantified spectrophotometrically.

Biochanin A and formononetin concentrations were established for samples collected from two greenhouse and two field harvests of a complete six-clone diallel cross. Analysis of variance by Griffing's combining-ability procedure indicated that the significant genetic variance for the isoflavones was primarily explained by general combining ability.

The upper leaflets of zigzag clover contained about 1/3 more biochanin A and formononetin than the lower ones, while the internodes contained about 1/10 as much formononetin as the leaflets, and only a trace of biochanin A.

Key Words: Isoflavones • Zigzag clover • Estrogenic activity

1 Contribution from the Departments of Agronomy and Animal Sciences, Purdue Agricultural Experiment Station, Lafayette, Ind. 47907, Journal Paper No. 3960. Part of a thesis presented by the senior author in partial fulfillment of the requirements for the Ph.D. degree. Supported in part by Public Health Service Research Grant No. U. I. 00279, National Center for Urban and Industrial Health.

2 Former Graduate Research Assistant in Agronomy (now with DeKalb AgResearch, Inc., Lubbock, Texas), Professor of Agronomy, and Professor of Animal Sciences, Purdue University, respectively.

Received for publication January 28, 1970.




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