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Variety-Specific Variants of Oxidative Enzymes from Soybean Seed¹

Three distinct electrophoretic patterns in acrylamide gel zymograms are associated with specific soybean varieties. The patterns represent at least two different enzymes, including peroxidase, hut collectively are called "INT-oxidases." The enzymes, extracted from dry soybean seeds, inhibit the reduction of colorless iodonitrotetrazolium-violet (INT) into the red INT-formazan and thus appear as clear bands on a red background.

Key Words: Glycine max (L.) Merrill • Electrophoresis • 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride • Isoenzymes • Peroxidase

² Research Botanist and Laboratory Technician, Field Crops and Animal Products Research Branch, Market Quality Research Division, Agricultural Research Service, U. S. Department of Agriculture, Beltsville, Maryland. 20705.

Received for publication January 30, 1970.