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Hybrids of Trifolium pratense x Trifolium pallidum¹

Triploid hybrids, 2n=22, were produced from crosses of autotetraploid Trifolium pratense L., 2n=28, x diploid T. pallidum Waldst. & Kit., 2n=16. We obtained 194 hybrids from 2,575 hand-pollinated florets. Many hybrids were vigorous, flowered profusely, and were intermediate to the parent Species in morphology. Chromosomal homology of the parental genomes was slight. At metaphase I the hybrids averaged 7.32 I, 6.34 II, 0.54 III and a very low frequency of multivalents containing more than three chromosomes. The hybrids were male- and female-sterile. A hypo-autotriploid plant of T. pratense>, 2n=20, was fertile and averaged 1.90 I, 2.89 II, and 4.08 III in microsporocytes at metaphase I.

Key Words: Triploid interspecific hybrid • Cytology • Chromosome pairing • Red clover • Hypo-autotriploid red clover

² Formerly Graduate Research Assistant (now Cytogeneticist, Ottawa Research Station, C. E. F., C. D. A., Ottawa, Canada) and Associate Professor of Agronomy.

Received for publication November 13, 1969.

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