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Published in Crop Sci 10:323-326 (1970)
© 1970 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Colorimetric Measurements of Purple Flower Color in Alfalfa as Affected by Variety, Soil pH, Soil Fertility, Light, and Seed Source1

Kevin P. Sheridan and Guy W. McKee2

Measurements of the color of purple flowers from 10 varieties of alfalfa were made on two types of instruments. These were (1) reflection device using tristimulus filters for comparison of intact petals and (2) a grating type spectrophotometer for comparison of aqueous extracts. Alfalfa plants of the same variety grown under several field, greenhouse, and growth chamber conditions varied significantly in intensity of purple flower color. Flowers from field grown plants were lighter in color than plants produced in the greenhouse and growth chamber. Differences in flower color associated with soil pH, soil fertility, and seed lot were minor. When produced and measured under standardized conditions, purple flowers from different inflorescences on the same plant did not vary significantly nor did flowers produced on the same plant at different time periods. Either reflectance readings or colorimetric measurements were useful in characterizing varieties.

Key Words: Varietal characterization • Varietal purity testing • Environmental affects

1 Authorized for publication as paper number 3282 in the journal series of the Pennsylvania Agr. Exp. Sta. This study was part of Northeast Regional Project, NEM-22, Facilitating the Marketing of Seed Through Improved Testing Procedures, and was supported in part by regional funds.

2 Graduate Assistant and Associate Professor, respectively, Department of Agronomy, The Pennsylvania State University. K. P. Sheridan is presently Research Officer, Agricultural Research Station, New South Wales, Department of Agriculture, Tamworth, N.S.W., Australia.

Received for publication August 6, 1969.




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