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Cytological and Morphological Investigations on the Grass Hemarthria altissima (Poir) Stapf et C.E. Hubb.

Two introductions of Hemarthria altissima, limpograss, from South Africa were diploid (2n = 18), one was tetraploid (2n = 36), and one was hexaploid (2n = 54). Pollen and embryo sac development were studied. Each of the accessions produced a high percentage of viable pollen. Addition of gibberellic acid to the medium gave a significant increase in in vitro pollen germination. The four strains were interpollinated and the ovaries were killed after 1, 2, and 4 hours incubation. Double fertilization occurred in all four accessions. No apomictic development was detected. The polyploids set seed after self pollination under terylene bags, but the diploids did not. One diploid and both polyploids yielded some seed under open pollination. These results indicate that Hemarthria altissima can be included in a conventional plant breeding program.

Key Words: Embryo sac development • Limpo-grass • Sexual reproduction • Gibberellic acid • Pollen germination

² Research Assistants and. Associate Professor, respectively, IFAS, University of Florida, Gainesville, Fla. 32601.

Received for publication December 31, 1969.